WASHINGTON — Conan O'Brien will receive the Mark Twain Prize on Sunday night at the John F. Kennedy Center for the Performing Arts, where the backstage drama of the host institution hangs over the celebration.

O'Brien, 61, was named the latest recipient of the award for lifetime achievement in comedy in mid-January, about three weeks before President Donald Trump upended the Kennedy Center by ousting the longtime president and the board chairman. Trump dismissed the board of directors and replaced them with loyalists, who then elected him as chairman.

As a Mark Twain recipient, O'Brien received tributes and testimonials from a star-studded collection of comics and celebrities. Many of them also took aim at the institution's uncertain future.

''I think it would be insane not to address the elephant in the room,'' comic Nikki Glaser said on the red carpet before the event began. ''It's in the air tonight. This night is about Conan, but it can be both.''

Once the festivities began, Stephen Colbert joked that the Kennedy Center had announced two new board members: Bashar Assad, the ousted president of Syria, and Skeletor, a fictional supervillain.

John Mulaney quipped that the entire building would soon be renamed ''the Roy Cohn Pavilion'' after one of Trump's mentors. And Sarah Silverman made multiple Trump references that cannot be printed.

Trump, in announcing the Kennedy Center changes, posted on social media that those who were dismissed "do not share our Vision for a Golden Age in Arts and Culture.''

How that vision takes shape remains to be seen, but Trump has spoken about wanting to book more Broadway shows there and floated the idea of granting Kennedy Center Honors status to actor Sylvester Stallone and singer-songwriter Paul Anka.

Into this maelstrom steps O'Brien, whose comedic persona has never been particularly political. The comic has always tended more toward goofiness and self-deprecation. But he has also leaned into sensitive societal issues at times. In 2011, O'Brien officiated a gay wedding live on his show, overseeing the marriage of his longtime costume designer Scott Cronick and his partner David Gorshein.

O'Brien vaulted into the spotlight from near-total obscurity in 1993 when he was chosen to replace David Letterman as host of ''Late Night'' despite no significant on-camera experience. The former Harvard Lampoon editor had spent the previous years as a writer for ''Saturday Night Live'' and ''The Simpsons,'' appearing on camera only as an occasional background extra in ''SNL'' skits.

He went on to host ''Late Night'' for 16 years, longer than any other host. O'Brien was later tapped to replace Jay Leno as host of ''The Tonight Show,'' but that experiment ended in public failure. After seven months of declining ratings, NBC executives brought Leno back for a new show that would bump ''The Tonight Show'' back. O'Brien refused to accept the move, leading to a public spat that ended with a multimillion-dollar payout for O'Brien and his staff to exit the network in early 2010.

O'Brien went on to host another talk show on the cable station TBS, while launching successful podcasts and travel shows. He is currently on a late-career elder statesman hot streak. His travel series, ''Conan O'Brien Must Go,'' drew popular and critical acclaim, with a second season coming. His recent gig hosting the Academy Awards was so well received that the producers announced they are bringing him back next year.

In the wake of Trump's takeover of the Kennedy Center, several artists, including the producers of ''Hamilton'' and actress and writer Issa Rae, announced they were canceling appearances at the venue.

Others have chosen to perform while making their sentiments known from the stage. Leftist comic W. Kamau Bell directly addressed the controversy in his performance just days after the shake-up. Earlier this month, cellist Erin Murphy Snedecor ended her set with a performance of the Woody Guthrie protest anthem ''All You Fascists Bound to Lose.''

Other comedians receiving the lifetime achievement award include both Letterman and Leno, along with George Carlin, Whoopi Goldberg, Bob Newhart, Carol Burnett, Bill Murray and Dave Chappelle.

The ceremony will be streamed on Netflix later this year. The date has not been announced.